Submitting Samples
Please contact the Sequencing Facility Manager, Ryan Percifield, when submitting samples. There are multiple options for getting samples to the Genomics Core Facility:
- Schedule an appointment to bring samples to the facility. Contact the lab manager, Ryan Percifield via e-mail or call at (304) 293-4542 / (304) 293-8051.
- If dropping samples off after hours, there is a box labeled "Genomics Core Drop Off" in room 5217 of the Life Sciences Building. Please provide and accompanying e-mail notifying the genomics core staff that samples were placed here so they can be processed as quickly as possible.
- Send samples by campus mail to Post Box 6057. In this case, samples should be properly packaged for shipping, and the facility manager should be notified in advance.
- Send samples by regular mail to:
- 53 Campus Drive
5109 Life Sciences Bldg
Morgantown, WV 26506 - Always ship samples so they arrive Monday through Friday. To avoid decay and delays in processing, schedule samples to arrive Monday-Friday and avoid Saturdays, Sundays, or Holidays.
- RNA : pack RNA in dry ice
-
DNA: pack DNA with ice packs
- If you are on campus, Ryan is often visiting between campus locations and can occasionally pick samples up.
Ordering Sequencing or Genotyping Service
This Genomics Core Facility website is where you should submit requests. Please upload a comma-delimited or tab-delimited text file that specifies sequencing information in the format listed below. Additionally, also upload a gel image if applicable. Below you’ll find information about proper formatting and example files.
Proper File Format
The purpose of the flat-file is to specify well information for your sample sequencing. The genetic sequencer uses a plate that contains 96-wells. Of those 96 available wells, your flat-file must specify which wells to use, their contents, and parameters. You can specify up to 96-wells per work order and thus per flat-file. You cannot use multiple flat-files for one order. If you need more than 96 wells, please submit multiple orders to do so.
The system expects the sequencing well information in lines and will ignore blank lines. It also expects well-specific information in columns as outlined below. The columns can be separated by commas or by tabs. Please see example flat files below to ensure accurate submission.
Here are the default required columns:
- Well
- Each well on the plate has a specific alpha-numeric value associated with
it. There are eight alpha-columns (A-H) and twelve numeric-rows (1-12) per
plate for a total of 96 wells (A01 through H12). The specification is
alpha-column first and numeric-row second, like A01.
- Begin with A01, B01, C01, ..., H01, A02, B02, so on and so forth
- Template Name
- The system can not read certain characters. Please do not use spaces, periods (.), parentheses ( ), commas (,), asterisks (*), dollar signs ($), etc. This will yield an error when running samples.
- When naming your templates please use only: letters, numbers, dashes (-) and underscores ( _ )
- Template Concentration (ng/uL)
- For amplicons, please submit samples up to 50 ng/uL.
- For plasmids, please submit samples around 250 ng/uL.
- Name of Primer
- Primer Concentration [uM]
- Please submit primers at 5 uM
- Vector Name
- Size of Insert (bp)
Blank wells: You can specify blank wells (in-between actual samples) if you wish. In order to do so, specify Blank or F for the Template Name in the file.